Deoxyribonucleotide synthesis in an Escherichia coli mutant (H 1491) which lacks ribonucleotide reductase subunit B2.

An iron-sensitive mutant of E. coli with a Mudl phage insertion in the nrdB gene lacks subunit B2 of the key enzyme of DNA synthesis, ribonucleotide reductase. Nevertheless, these cells are capable of growing in minimal media under anaerobic conditions, indicating a second enzyme or pathway for deoxyribonucleotide synthesis. We here show that ribonucleotide… CONTINUE READING