Deoxyribonucleoside triphosphate pools and growth of glutathione-depleted 3T6 mouse fibroblasts.

@article{Spyrou1996DeoxyribonucleosideTP,
  title={Deoxyribonucleoside triphosphate pools and growth of glutathione-depleted 3T6 mouse fibroblasts.},
  author={Glannis Spyrou and Arne Holmgren},
  journal={Biochemical and biophysical research communications},
  year={1996},
  volume={220 1},
  pages={
          42-6
        }
}
Buthionine sulfoximine (BSO) selectively blocks g-glutamylcysteine synthetase and thereby depletes cells of glutathione (GSH). In cultures of exponentially growing 3T6 mouse fibroblasts, 0.1 mM BSO rapidly stopped GSH synthesis after treatment for 12 hours. The GSH-depleted cells grew as well as control 3T6 cells with no decrease in DNA synthesis. Furthermore, the pools of deoxyribonucleoside triphosphates (dNTPs), typically tightly regulated in cultured cells, did not change in size… CONTINUE READING
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