Deoxyribonucleic acid sequence mapping on metaphase chromosomes by immunoelectron microscopy.

@article{Narayanswami1989DeoxyribonucleicAS,
  title={Deoxyribonucleic acid sequence mapping on metaphase chromosomes by immunoelectron microscopy.},
  author={Sandya Narayanswami and Karen Lundgren and Barbara A. Hamkalo},
  journal={Scanning microscopy. Supplement},
  year={1989},
  volume={3},
  pages={65-76}
}
Nucleic acid sequences can be localized on chromosomes in the electron microscope after hybridization with a biotinylated DNA probe followed by detection with a primary antibiotin antibody and a secondary antibody coupled to colloidal gold. Hybridization probes can also be labelled with alternative ligands such as N-acetoxy-2-acetylaminofluorene (AAF), Dinitrophenyl-dUTP and Digoxigenin-dUTP. Multiple labelling is possible if these differently modified DNA probes are used in conjunction with… CONTINUE READING