Density-dependent regulation of growth of BSC-1 cells in cell culture: growth inhibitors formed by the cells.

@article{Holley1978DensitydependentRO,
  title={Density-dependent regulation of growth of BSC-1 cells in cell culture: growth inhibitors formed by the cells.},
  author={Robert W. Holley and R W Armour and J H Baldwin},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1978},
  volume={75 4},
  pages={1864-6}
}
Inhibitors formed by a monkey epithelial cell line, BSC-1, play an important role in limiting growth at high cell densities. At least three inhibitors are formed: lactic acid, ammonia, and an unidentified inhibitor that may be an unstable protein. The unidentified inhibitor is destroyed by shaking the conditioned medium, by bubbling gas through the medium, or by heating or storing the medium in the absence of cells. The concentrations of lactic acid and ammonia that accumulate in conditioned… CONTINUE READING