Densitometric quantification of brain gangliosides separated by two-dimensional thin layer chromatography.

@article{Chigorno1982DensitometricQO,
  title={Densitometric quantification of brain gangliosides separated by two-dimensional thin layer chromatography.},
  author={Vanna Chigorno and Sandro Sonnino and Riccardo Ghidoni and Guido Tettamanti},
  journal={Neurochemistry international},
  year={1982},
  volume={4 5},
  pages={397-404}
}
A procedure for accurate densitometric quantification of gangliosides separated by two-dimensional thin layer chromatography is reported. The procedure was set up employing 9 different pure gangliosides and was applied to the analysis of calf and pig brain gangliosides. Silica gel high performance thin layer plates, 10 x 10 cm. were two-dimensionally developed at 18-20 C with the following solvents: chloroform methanol 0.2% aqueous CaCl(2), 50/40/10 by volume, for the first run; n-propanol 17 M… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 11 extracted citations

Why Is N-Glycolylneuraminic Acid Rare in the Vertebrate Brain?

Topics in current chemistry • 2015
View 7 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…