Denitrification, a Novel Type of Respiratory Metabolism in Fungal Mitochondrion*

@article{Kobayashi1996DenitrificationAN,
  title={Denitrification, a Novel Type of Respiratory Metabolism in Fungal Mitochondrion*},
  author={M. Kobayashi and Y. Matsuo and A. Takimoto and S. Suzuki and F. Maruo and H. Shoun},
  journal={The Journal of Biological Chemistry},
  year={1996},
  volume={271},
  pages={16263 - 16267}
}
Subcellular localization and coupling to ATP synthesis were investigated with respect to the denitrifying systems of two fungi, Fusarium oxysporum and Cylindrocarpon tonkinense. Dissimilatory nitrate reductase of F. oxysporum or nitrite reductase of C. tonkinense could be detected in the mitochondrial fraction prepared from denitrifying cells of each fungus. Fluorescence immunolocalization, cofractionation with mitochondrial marker enzymes, and cytochromes provided evidence that the… Expand
A Possible Role of NADPH-Dependent Cytochrome P450nor Isozyme in Glycolysis under Denitrifying Conditions
TLDR
The results suggest that the NADP-dependent pentose phosphate cycle acts predominantly in C. tonkinense as the glycolysis pathway under the denitrifying conditions, which was confirmed by the observation that glucose induced enzyme activities involved in the cycle were confirmed. Expand
Response to Hypoxia, Reduction of Electron Acceptors, and Subsequent Survival by Filamentous Fungi
  • N. Takaya
  • Biology, Medicine
  • Bioscience, biotechnology, and biochemistry
  • 2009
TLDR
This review examines the metabolic mechanisms used by filamentous fungi under oxygen-limited (hypoxic) conditions and suggests that ammonia fermentation and nitrate utilization mechanisms probably share a nitrate-reducing mechanism. Expand
Fungal denitrification and nitric oxide reductase cytochrome P450nor
TLDR
Fungal denitrification is often accompanied by a unique phenomenon, co-denitrification, in which a hybrid N2 or N2O species is formed upon the combination of nitrogen atoms of nitrite with a nitrogen donor (amines and imines). Expand
Pyruvate : NADP+ oxidoreductase from the mitochondrion of Euglena gracilis and from the apicomplexan Cryptosporidium parvum: a biochemical relic linking pyruvate metabolism in mitochondriate and amitochondriate protists.
TLDR
Findings suggest a common ancestry of PFO in amitochondriate protists with Euglena mitochondrial PNO and Cryptosporidium PNO, consistent with the view that eukaryotic PFO domains are biochemical relics inherited from a facultatively anaerobic, eubacterial ancestor of mitochondria and hydrogenosomes. Expand
Fungal Ammonia Fermentation, a Novel Metabolic Mechanism That Couples the Dissimilatory and Assimilatory Pathways of Both Nitrate and Ethanol
TLDR
The results showed that the fungus ferments ammonium via coupled dissimilatory and assimilatory mechanisms. Expand
Cytochrome p450nor, a novel class of mitochondrial cytochrome P450 involved in nitrate respiration in the fungus Fusarium oxysporum.
TLDR
It is shown that one of the isoforms of P450nor (P450norA) is in anaerobic mitochondrion of F. oxysporum and acts as nitric oxide reductase, suggesting the involvement of P 450nor in nitrate respiration in mitochondria. Expand
Ammonia Fermentation, a Novel Anoxic Metabolism of Nitrate by Fungi*
TLDR
This fungus is the first eukaryotic, facultative anaerobe known to express one of three distinct metabolic energy mechanisms closely depending on environmental O2 tension, and it is showed that ammonia fermentation occurs in many other fungi that are common in soil, suggesting that facultativeAnaerobes are widely distributed among fungi that have been considered aerobic organisms. Expand
Dissimilatory nitrate reduction metabolisms and their control in fungi.
  • N. Takaya
  • Chemistry, Medicine
  • Journal of bioscience and bioengineering
  • 2002
TLDR
Fungal nitric oxide reductase uses NADH as the direct electron donor in contrast to bacterial systems and thus might function in regeneration of NAD+ and detoxification of the toxic radical, nitricoxide. Expand
Eukaryotic pyruvate formate lyase and its activating enzyme were acquired laterally from a Firmicute.
TLDR
The comprehensively searched for homologs of Pfl and Pfla in publicly available large-scale eukaryotic genomic and cDNA sequencing data indicate that the Pfl pathway was first acquired by lateral gene transfer into a eUKaryotic lineage most probably from a firmicute bacterial lineage and that it has since been spread across diverse eukarianotic groups by more recent eukARYote-to-eukaryote transfer events. Expand
Denitrification by the Fungus Fusarium oxysporum Involves NADH-Nitrate Reductase
Fusarium oxysporum JCM11502 expresses a denitrifying (nitrate (NO3 −)-respiring) mechanism and can thrive under oxygen (O2) limitation. The fungus reduces NO3 − to nitrite at the initial step ofExpand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 15 REFERENCES
Denitrification by the fungus Cylindrocarpon tonkinense: anaerobic cell growth and two isozyme forms of cytochrome P-450nor
TLDR
The lack of nitrite-dependent N2O formation and/or cell growth during the anaerobic culture was not affected by further addition of ammonium ions but was suppressed by respiration inhibitors such as rotenone or antimycin, suggesting that denitrification plays a physiological role in respiration. Expand
Denitrification by the fungus Fusarium oxysporum and involvement of cytochrome P-450 in the respiratory nitrite reduction.
TLDR
This work has presented the first demonstration that a eukaryote exhibits a marked denitrifying ability, and the first instance of a cytochrome P-450 that is involved in a reducing reaction with a distinct physiological significance against a hydrophilic, inorganic substrate. Expand
The Copper-containing Dissimilatory Nitrite Reductase Involved in the Denitrifying System of the Fungus Fusarium oxysporum(*)
TLDR
The intracellular localization of Cu-NiR was investigated, and it was suggested that the Cu- NiR localizes in an organelle such as mitochondrion, the first isolation of dissimilatory NiR from a eukaryote. Expand
Cytochrome P-450 55A1 (P-450dNIR) acts as nitric oxide reductase employing NADH as the direct electron donor.
TLDR
The present results demonstrated not only the unique reaction catalyzed by P- 450, but also the first P-450-dependent reaction where the electron transport from NAD(P)H to P-550 is not supported by other components. Expand
Nitric oxide reductase cytochrome P-450 gene, CYP 55, of the fungus Fusarium oxysporum containing a potential binding-site for FNR, the transcription factor involved in the regulation of anaerobic growth of Escherichia coli.
TLDR
Northern blot analyses showed that both nitrate and nitrite are the actual inducers of P-450nor and that its expression is predominantly regulated at the transcriptional level, raising the interesting possibility that the expression of the fungal denitrification system is regulated, as in the case of bacterial nitrate respiration, by a set of mechanisms. Expand
Spectroscopic and Kinetic Studies on Reaction of Cytochrome P450nor with Nitric Oxide
TLDR
It is suggested that in the catalytic cycle, decomposition of the intermediate is fairly accelerated by free NO, resulting in such a fast turnover as to justify the proposed mechanism of the NO reduction by P450nor. Expand
Nucleotide sequence of the unique nitrate/nitrite-inducible cytochrome P-450 cDNA from Fusarium oxysporum.
TLDR
A cDNA clone for the nitrate/nitrite-inducible cytochrome P-450 of the fungus Fusarium oxysporum was isolated by an immunoscreening method and revealed a polypeptide of 403 amimo acid residues, which was shown to contain the full-length sequence of the fungal P- 450. Expand
Soluble, nitrate/nitrite‐inducible cytochrome P‐450 of the fungus, Fusarium oxysporum
TLDR
It is reported here that the P‐450 in the soluble fraction was induced only when nitrate or nitrite was added to the growth medium, whereas the microsomal P‐ 450 was synthesized regardless of the medium compositions. Expand
Paracoccus denitrificans and the evolutionary origin of the mitochondrion
It is demonstrated that Paracoccus denitrificans resembles a mitochondrion more closely than do other bacteria, in that it effectively assembles in a single organism those features of theExpand
Expression of the fungal cytochrome P-450nor cDNA in Escherichia coli.
TLDR
The expression of the unique cytochrome P-450, nitric oxide reductase (P-450nor), in Escherichia coli by utilizing P- 450nor cDNA and the expression vector pKK233-2 or pUC19 provided conclusive evidence that the enzymatic reaction depends solely on P-550nor without support of any other electron transferring components. Expand
...
1
2
...