Dengue virus type 2 recognizes the carbohydrate moiety of neutral glycosphingolipids in mammalian and mosquito cells.

Abstract

Dengue viruses infect cells by attaching to a surface receptor which remains unknown. The putative receptor molecules of dengue virus type 2 on the surface of mosquito (AP-61) and mammalian (LLC-MK2) cell lines were investigated. The immunochemical detection and structural analysis of carbohydrates demonstrated that the neutral glycosphingolipids, L-3 (GlcNAcβ1-3Manβ1-4Glcβ1-1'Cer) in AP-61 cells, and nLc(4) Cer (Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1'Cer) in LLC-MK2 cells were recognized by the virus. These findings strongly suggest that neutral glycosphingolipids share the key determinant for virus binding and that the β-GlcNAc residue may play an important role in dengue virus binding to the host cell surface.

DOI: 10.1111/j.1348-0421.2010.00293.x
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@article{Wichit2011DengueVT, title={Dengue virus type 2 recognizes the carbohydrate moiety of neutral glycosphingolipids in mammalian and mosquito cells.}, author={Sineewanlaya Wichit and Akanitt Jittmittraphap and Kazuya I P J Hidari and Butsaya Thaisomboonsuk and Songsak Petmitr and Sukathida Ubol and Chie Aoki and Saki Itonori and Koichi Morita and Takashi Suzuki and Yasuo Suzuki and Wipawee Jampangern}, journal={Microbiology and immunology}, year={2011}, volume={55 2}, pages={135-40} }