Dengue virus strain DEN2 16681 utilizes a specific glycochain of syndecan-2 proteoglycan as a receptor.

Abstract

Dengue virus (DENV) causes fever and severe haemorrhagic symptoms in humans. The DEN2 16681 strain, derived from a dengue haemorrhagic fever patient, has been widely used in studies related to DENV pathogenesis, such as mouse and non-human primate haemorrhagic models and human vascular endothelial-cell permeability. To clarify the entry mechanism of the 16681 strain, we characterized a novel cell receptor for this strain. Our two major findings were as follows: firstly, the SDC2 membrane protein was an effective DEN2 16681 receptor in a cloned K562 cell line. Secondly, a heparan sulfate (HS) glycochain (of four glycochains in SDC2) is the specific binding site of DENV and seems to be involved in tissue-culture adaptation. Our findings present an entry mechanism that could be implicated for DENV adaptation and HS-mediated DENV infection.

DOI: 10.1099/vir.0.037853-0
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@article{Okamoto2012DengueVS, title={Dengue virus strain DEN2 16681 utilizes a specific glycochain of syndecan-2 proteoglycan as a receptor.}, author={Kenta Okamoto and Hitomi Kinoshita and Maria Del Carmen Parquet and Muhareva Raekiansyah and Daisuke Kimura and Katsuyuki Yui and Mohammed Alimul Islam and Futoshi Hasebe and Kouichi Morita}, journal={The Journal of general virology}, year={2012}, volume={93 Pt 4}, pages={761-70} }