Dendritic cells in chronic myelomonocytic leukaemia

  title={Dendritic cells in chronic myelomonocytic leukaemia},
  author={Slavica Vuckovic and D. B. Fearnley and Sarah P. Gunningham and Ruth Spearing and William Nigel Patton and Derek N. J. Hart},
  journal={British Journal of Haematology},
Blood dendritic cells (DC) differentiate in vitro via two separate pathways: either directly from blood DC precursors (DCp) or from CD14+ monocytes. In chronic myelomonocytic leukaemia (CMML) abnormal bone marrow precursors contribute to blood monocyte development but DC development has not been studied previously. Monocytes comprised 60% of blood MNC in 15 CMML patients studied, compared with 20% in 16 age‐matched controls. The increase in blood monocytes was accompanied by a reciprocal… 

Phenotypic and functional deficiencies of leukaemic dendritic cells from patients with chronic myeloid leukaemia

Data indicated that the maturation response of leukaemic monocyte‐derived DC to a natural stimulus like LPS is abnormal and may be caused by an aberrant TNF‐α response in these cells, and TNF-α alone or in combination with pro‐inflammatory and T‐cell stimulatory cytokines should be considered as an adjuvant for DC‐based immunotherapy in CML.

Circulating blood dendritic cells from myeloid leukemia patients display quantitative and cytogenetic abnormalities as well as functional impairment.

Evidence is provided that DC subsets in vivo may be affected by leukemogenesis and may contribute to leukemia escape from immune control.

Generation of Dendritic Cells from Human Chronic Myelomonocytic Leukemia Cells in Fetal Calf Serum-Free Medium

It is demonstrated that peripheral blood cells of patients with chronic myelomonocytic leukemia can be induced to acquire DC characteristics and generation of CMML-derived DCs may be used as a cellular vaccine to induce anti-tumor immunity in patients with CMML.

Capacity of Monocytes from Patients with Chronic Myelomonocytic Leukemia to Differentiate into Macrophages and Multinucleated Giant Cells

CMML-Mo could generate dendritic cells (DC), but not multinucleated giant cells (MGC) and the ability to differentiate into MΦ and MGC, but not DC, is suggested.

The effect of LIGHT in inducing maturation of monocyte-derived dendritic cells from MDS patients

It is concluded that both LIGHT and CD40L are immunoregulating factors that induce monocyte-derived iDCs from MDS patients to undergo maturation resulting in increased antigen presentation and T-cell activation.

CD1c(+) myeloid dendritic cells in myeloid neoplasia

Findings indicate CD1c(+) MDC elevations are not uncommon in myeloid leukemias and are associated with CMML and AML, particularly AML with inv(16) cytogenetic abnormality.

Circulating myeloid and lymphoid precursor dendritic cells are clonally involved in myelodysplastic syndromes

Data suggest that myeloid and lymphoid pDC share a common precursor, and whether reduced peripheral blood counts of pDC contribute to the immunological abnormalities observed in MDS remains to be investigated.



Proliferating dendritic cell progenitors in human blood

Large numbers of DC progenitors are observed in cord blood and in adult blood from healthy donors, which should facilitate future studies of their Fc epsilon RI and CD4 receptors, and their use in stimulating T cell-mediated resistance to viruses and tumors.

Monitoring human blood dendritic cell numbers in normal individuals and in stem cell transplantation.

A statistically significant decrease in the blood DC counts in individuals at the time of blood stem cell harvest and in patients with acute illnesses, including allogeneic bone marrow transplant (BMT) recipients with acute graft-versus-host disease (aGVHD).

Dendritic cells as the terminal stage of monocyte differentiation.

MO are precursors of Mphi as well as of DC, with each cell type having the capability to convert into the other until late in the differentiation/maturation process, and the cytokine environment and the presence of differentiation and other stimulatory signals may be the "final decision-making factors" determining whether these cells will acquire DC or Mphi characteristics and function.

CD14+ blood monocytes can differentiate into functionally mature CD83+ dendritic cells.

  • L. ZhouT. Tedder
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1996
The identification of specific culture conditions that generate large numbers of dendritic cells from purified monocytes uncovers an important step in dendrite cell maturation that will allow the further characterization of their role in autoimmune diseases, graft rejection, and human immunodeficiency virus infection.

Generation of CMRF-44+ monocyte-derived dendritic cells: insights into phenotype and function.

It is concluded that the MoAb CMRF-44 identifies both intermediate and fully mature stages of monocytes-DC differentiation and may be a useful marker in establishing the optimal timing for antigen loading of in vitro-generated monocyte-derived DCs.

CD 14 + blood monocytes can differentiate into functionally mature CD 83 + dendritic cells ( cytokines / antigen presentation / mixed leukocyte reaction )

Plastic-adherent blood monocytes cultured for 7 days with granulocyte-monocyte colony-stimulating factor, interleukin 4, and tumor necrosis factor a were shown to differentiate into CDla+ CD83+ dendritic cells, which were the most potent stimulatory cells in allogeneic mixed leukocyte reactions.

Dendritic cells and macrophages can mature independently from a human bone marrow-derived, post-colony-forming unit intermediate.

Normal CD34+ BM precursors can generate a post-CFU bipotential intermediate that will develop along the dendritic cell pathway when macrophages are removed and GM-CSF and TNF-alpha are provided, and can differentiate along a macrophage pathway when recultured with or without M- CSF.

The novel subset of CD14+/CD16+ blood monocytes is expanded in sepsis patients.

Three-color immunofluorescence shows that the CD14+/CD16+ monocytes in septicemia patients when compared with theCD14++ monocytes exhibit a higher level of class II antigen and a lower level of CD11b and CD33 antigens, consistent with a more mature nature of the CD 14+/ CD16+ cells.

The novel subset of CD14+/CD16+ blood monocytes is expanded in sepsis patients

Three-color immunofluorescence shows that the CD14+/CD16+ monocytes in septicemia patients when compared with theCD14++ monocytes exhibit a higher level of class II antigen and a lower level of CD11b and CD33 antigens, consistent with a more mature nature of the CD 14+/ CD16+ cells.

The phenotype of freshly isolated and cultured human bone marrow allostimulatory cells: possible heterogeneity in bone marrow dendritic cell populations.

The data suggest that there are at least two phenotypically diverse forms of potent allostimulatory cells in the lineage-negative fraction of human BM, at least some of which express the early haemopoietic precursor antigens CD34 or CD33.