Dendritic cell culture: a simple closed culture system using ficoll, monocytes, and a table-top centrifuge.

@article{Celluzzi2003DendriticCC,
  title={Dendritic cell culture: a simple closed culture system using ficoll, monocytes, and a table-top centrifuge.},
  author={Christina M Celluzzi and Craig C. Welbon},
  journal={Journal of hematotherapy & stem cell research},
  year={2003},
  volume={12 5},
  pages={575-85}
}
Dendritic cells (DCs) are potent antigen-presenting cells involved in the induction of T cell-mediated immune responses and as such have emerged as important candidates for cellular-based therapies. Critical to safe clinical use is the easy manipulation of DCs and their precursors in a closed system. We have developed a serum-free, closed culture system applying a simple wash-Ficoll centrifugation method to reduce platelet and red blood cell (RBC) contamination. This procedure optimized… CONTINUE READING