Denaturing protein immunoprecipitation from Mammalian cells.

@article{Tansey2007DenaturingPI,
  title={Denaturing protein immunoprecipitation from Mammalian cells.},
  author={William P Tansey},
  journal={CSH protocols},
  year={2007},
  volume={2007},
  pages={
          pdb.prot4619
        }
}
INTRODUCTIONThis protocol describes a denaturing immunoprecipitation (IP) for mammalian cells. Where possible, we prefer to use denaturing IPs to recover labeled proteins from pulse-chase experiments. The use of denaturing IPs reduces background considerably. 

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