Denaturing gradient gel electrophoresis (DGGE): a rapid and sensitive technique to screen nucleotide sequence variation in populations.

@article{Miller1999DenaturingGG,
  title={Denaturing gradient gel electrophoresis (DGGE): a rapid and sensitive technique to screen nucleotide sequence variation in populations.},
  author={Kristina M. Miller and Tobi J. Ming and Angela D. Schulze and Ruth E. Withler},
  journal={BioTechniques},
  year={1999},
  volume={27 5},
  pages={
          1016-8, 1020-2, 1024 passim
        }
}
We describe a rapid and sensitive method for the detection of nucleotide sequence variation that can be used for large-scale screening of population markers. Denaturing gradient gel electrophoresis (DGGE) detects sequence variants of amplified fragments by the differences in their melting behavior. DGGE detects most single-base substitutions when carried out on products amplified with a primer to which a GC clamp has been added. Although DGGE has been primarily used for the detection of limited… CONTINUE READING

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