Denaturation of the simian virus 40 origin of replication mediated by human replication protein A.

@article{Iftode1997DenaturationOT,
  title={Denaturation of the simian virus 40 origin of replication mediated by human replication protein A.},
  author={Cristian Iftode and James A. Borowiec},
  journal={Molecular and cellular biology},
  year={1997},
  volume={17 7},
  pages={
          3876-83
        }
}
The initiation of simian virus 40 (SV40) replication requires recognition of the viral origin of replication (ori) by SV40 T antigen, followed by denaturation of ori in a reaction dependent upon human replication protein A (hRPA). To understand how origin denaturation is achieved, we constructed a 48-bp SV40 "pseudo-origin" with a central 8-nucleotide (nt) bubble flanked by viral sequences, mimicking a DNA structure found within the SV40 T antigen-ori complex. hRPA bound the pseudo-origin with… CONTINUE READING

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