Denaturation and renaturation of viral ribonucleic acid. I. Annealing R17 ribonucleic acid with denatured replicative form or with denatured replicative intermediate.

Abstract

Purified replicative form (RF) and replicative intermediate (RI) prepared from Escherichia coli infected with R17 were denatured in 0.15 m NaCl, 0.015 m sodium citrate containing 65% dimethylsulfoxide. Denaturation of RF or RI was demonstrated spectrophotometrically, chromatographically, and by sedimentation analysis. Denatured RF or RI was annealed by… (More)

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