Demystifying SP cell purification: viability, yield, and phenotype are defined by isolation parameters.

@article{Montanaro2004DemystifyingSC,
  title={Demystifying SP cell purification: viability, yield, and phenotype are defined by isolation parameters.},
  author={Federica Montanaro and Kalliopi Liadaki and Jaclyn Schienda and Alan Flint and Emanuela Gussoni and Louis M. Kunkel},
  journal={Experimental cell research},
  year={2004},
  volume={298 1},
  pages={144-54}
}
Side population (SP) cells isolated from bone marrow, skeletal muscle, and skin have been shown to engraft in dystrophic muscle. However, there have been questions on the phenotypical heterogeneity, tissue of origin, and relationships among SP cell populations extracted from different tissues. Studies on bone marrow SP cells have followed a consistent protocol for their isolation and results obtained are concordant. In contrast, protocols for the isolation of muscle SP cells vary greatly, and… CONTINUE READING

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