Demonstration that the shear force required to separate short double-stranded DNA does not increase significantly with sequence length for sequences longer than 25 base pairs.

@article{Hatch2008DemonstrationTT,
  title={Demonstration that the shear force required to separate short double-stranded DNA does not increase significantly with sequence length for sequences longer than 25 base pairs.},
  author={Kristi Adamson Hatch and Claudia B Danilowicz and Vincent W. Coljee and Mara G. Prentiss},
  journal={Physical review. E, Statistical, nonlinear, and soft matter physics},
  year={2008},
  volume={78 1 Pt 1},
  pages={011920}
}
We have measured the shear force for short double-stranded DNA sequences pulled by either the 3'3' or 5'5' ends and find that the shear force is independent of the pulling technique. For the 50% GC sequences examined, the force is a linear function of DNA length up to 20 base pairs (bp); however, we show that, as predicted by deGennes, the shear force… CONTINUE READING