Demonstration that monocytes rather than lymphocytes are the insulin-binding cells in preparations of humah peripheral blood mononuclear leukocytes: implications for studies of insulin-resistant states in man.

@article{Schwartz1975DemonstrationTM,
  title={Demonstration that monocytes rather than lymphocytes are the insulin-binding cells in preparations of humah peripheral blood mononuclear leukocytes: implications for studies of insulin-resistant states in man.},
  author={Ronald H. Schwartz and Angelo Raffaele Bianco and Barry S. Handwerger and C Ronald Kahn},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1975},
  volume={72 2},
  pages={474-8}
}
Insulin receptors have been demonstrated on mononuclear leukocytes prepared by centrifugation of buffy coats from normal blood donors on Ficoll-Hypaque gradients. The cell type that specifically binds insulin in this mixture of lymphocytes and monocytes has never been clearly identified, although it was assumed to be the lymphocyte since this cell constitutes about 80% of the population. In the present studies, insulin-binding assays were performed on the mononuclear leukocyte preparation… CONTINUE READING