Demonstration of the specificity of poliovirus encapsidation using a novel replicon which encodes enzymatically active firefly luciferase.

@article{Porter1998DemonstrationOT,
  title={Demonstration of the specificity of poliovirus encapsidation using a novel replicon which encodes enzymatically active firefly luciferase.},
  author={Donna C Porter and David C Ansardi and Jin Wang and Sylvia McPherson and Zina Moldoveanu and Casey D. Morrow},
  journal={Virology},
  year={1998},
  volume={243 1},
  pages={1-11}
}
The specificity of poliovirus encapsidation has been studied using a novel chimeric genome in which the gene encoding firefly luciferase has been substituted for the VP2-VP3-VP1 genes of the poliovirus capsid (P1) gene. Transfection of RNA transcribed in vitro from this genome resulted in a VP4-luciferase fusion protein which retained luciferase enzyme activity. Since the detection of enzyme activity was dependent upon replication of the transfected RNA genome, we refer to these genomes as… CONTINUE READING
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