Demonstration of specific binding sites for pituitary adenylate cyclase activating polypeptide (PACAP) in rat astrocytes.

@article{Tatsuno1990DemonstrationOS,
  title={Demonstration of specific binding sites for pituitary adenylate cyclase activating polypeptide (PACAP) in rat astrocytes.},
  author={Ichiro Tatsuno and Paul E. Gottschall and Katalin K{\"o}ves and Akira Arimura},
  journal={Biochemical and biophysical research communications},
  year={1990},
  volume={168 3},
  pages={1027-33}
}
The high and low affinity binding sites for PACAP were identified in rat astrocytes using [125I]PACAP27 as the labeled ligand. Scatchard analysis of displacement of the bound tracer by unlabeled PACAP27 indicated the existence of two classes of binding sites, with the dissociation constant (Kd) = 1.22 +/- 0.4 nM, the binding maximal capacity (Bmax) = 821 +/- 218 fmols/mg protein for the high affinity binding site, and Kd = 0.59 +/- 0.06 microM, Bmax = 563 +/- 12 pmols/mg protein for the low… CONTINUE READING
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