Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments

Abstract

BACKGROUND Human interactome is predicted to contain 150,000 to 300,000 protein-protein interactions, (PPIs). Protein-fragment complementation assay (PCA) is one of the most widely used methods to detect PPI, as well as Förster resonance energy transfer (FRET). To date, successful applications of firefly luciferase (Fluc)-based PCA have been reported in… (More)
DOI: 10.1186/1472-6750-13-31

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@inproceedings{OhmuroMatsuyama2013DemonstrationOP, title={Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments}, author={Yuki Ohmuro-Matsuyama and Chan-I Chung and Hiroshi Ueda}, booktitle={BMC biotechnology}, year={2013} }