Demonstration of nuclear compartmentalization of glutathione in hepatocytes.

  title={Demonstration of nuclear compartmentalization of glutathione in hepatocytes.},
  author={Giorgio Bellomo and Mariapia Vairetti and Lucia Anna Stivala and Francesca Mirabelli and Plinio Richelmi and Sten Orrenius},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={89 10},
The intracellular distribution of glutathione (GSH) in cultured hepatocytes has been investigated by using the compound monochlorobimane (BmCl), which interacts specifically with GSH to form a highly fluorescent adduct. Image analysis of BmCl-labeled hepatocytes predominantly localized the fluorescence in the nucleus; the nuclear/cytoplasmic concentration gradient was approximately three. This concentration gradient was collapsed by treatment of the cells with ATP-depleting agents. The uneven… CONTINUE READING