Demonstration of non-linear detection in ELISA resulting in up to 1000-fold too high affinities of fibrinogen binding to integrin alpha IIb beta 3.


To clarify the question as to why different solid-phase assays yield different results in terms of interaction strength, we used fibrinogen binding to immobilized alpha IIb beta 3 integrin as a test system. A classical 'three step' enzyme-linked (ELISA), a 'two step' biotin enzyme-linked streptavidin and a 'one step' radioligand assay were compared under… (More)


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