Demonstration of link protein in proteoglycan aggregates from human intervertebral disc.

@article{Tengblad1984DemonstrationOL,
  title={Demonstration of link protein in proteoglycan aggregates from human intervertebral disc.},
  author={A. Tengblad and Richard H. Pearce and B J Grimmer},
  journal={The Biochemical journal},
  year={1984},
  volume={222 1},
  pages={85-92}
}
Proteoglycan aggregates free of non-aggregating proteoglycan have been prepared from the annuli fibrosi and nuclei pulposi of intervertebral discs of three human lumbar spines by extraction with 4M-guanidinium chloride, associative density gradient centrifugation, and chromatography on Sepharose CL-2B. The aggregate (A1-2B.V0) was subjected to dissociative… CONTINUE READING