Demonstration of light chain restricted clonal B-lymphoid infiltrates in archival bone marrow trephines by quantitative real-time polymerase chain reaction.

@article{Lehmann2001DemonstrationOL,
  title={Demonstration of light chain restricted clonal B-lymphoid infiltrates in archival bone marrow trephines by quantitative real-time polymerase chain reaction.},
  author={Ulrich Lehmann and Oliver Bock and Florian Peter L{\"a}nger and Hans Kreipe},
  journal={The American journal of pathology},
  year={2001},
  volume={159 6},
  pages={2023-9}
}
Assessment of clonality either by demonstrating light chain restriction or showing monoclonal immunoglobulin gene rearrangement is a valuable and indispensable adjunct to diagnosis in hematopathology. The study of light chain restriction by immunohistochemistry on archival material is hampered by a very low sensitivity especially regarding low grade lymphomas of B cell origin. DNA rearrangement studies of the immunoglobulin locus do improve sensitivity markedly but for lymphomas of follicle… CONTINUE READING