Demonstration of four immunoassay formats using the array biosensor.

  title={Demonstration of four immunoassay formats using the array biosensor.},
  author={Kim E. Sapsford and Paul T. Charles and Charles Patterson and Frances S. Ligler},
  journal={Analytical chemistry},
  volume={74 5},
The ability of a fluorescence-based array biosensor to measure and quantify the binding of an antigen to an immobilized antibody has been demonstrated using the four different immunoassay formats: direct, competitive, displacement, and sandwich. A patterned array of antibodies specific for 2,4,6-trinitrotoluene (TNT) immobilized onto the surface of a planar waveguide and used to measure signals from different antigen concentrations simultaneously. For direct, competitive, and displacement… CONTINUE READING

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