Demonstration of different metal ion-induced calcineurin conformations using a monoclonal antibody.

@article{Matsui1985DemonstrationOD,
  title={Demonstration of different metal ion-induced calcineurin conformations using a monoclonal antibody.},
  author={H. Matsui and C. Pallen and A. Adachi and J. Wang and P. H. Lam},
  journal={The Journal of biological chemistry},
  year={1985},
  volume={260 7},
  pages={
          4174-9
        }
}
It has been suggested that calcineurin, a calmodulin-stimulated phosphatase, may exist in different metal ion-dependent conformational states (Pallen, C.J., and Wang, J. H. (1984) J. Biol. Chem. 259, 6134-6141). Evidence in favor of this hypothesis comes from studies involving a monoclonal antibody, VA1, which is specific for the small (beta) subunit of calcineurin. This antibody inhibits Ni2+-stimulated but not Mn2+-stimulated phosphatase activity against p-nitrophenyl phosphate and… Expand
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