Demonstration of acid phosphatase activity in the normal rat jejunum by means of electron microscopy.

  title={Demonstration of acid phosphatase activity in the normal rat jejunum by means of electron microscopy.},
  author={K. Ogawa and Y. Shinonaga and Y. Saito},
  journal={Okajimas folia anatomica Japonica},
The electron microscopic demonstration of acid phosphatase (ACP) activity was first tried by Sheldon et al. (1955) who applied G o m o r is method on the osmium-fixed mouse jejunum. Their method is, however, unnecessarily intricate and yet the preservation of fine structural details of epithelial cells is not successfully good. Holt (1959) recommended the use of cold formol-calcium fixation for the preservation of both ACP activity and morphological details in the light microscopic… Expand
Immunocytochemical localization of acid phosphatase in rat liver.
Localization of acid phosphatase (ACPase) in rat liver was investigated by immunocytochemical techniques and indicates that ACPase is present in endosomes and in a restricted area of plasma membrane, as well as in the lysosomal system. Expand
Ultrastructural localization of acid phosphatase activity in the small intestinal absorptive cells of adult rats
  • K. Ono
  • Biology, Medicine
  • Histochemistry
  • 2004
It was assumed that both the Golgi apparatus and GERL produce some lysosomes in the duodenal and jejunal absorptive cells, but only GERL does so in the ileum, because of an unexpectedly high number of lysOSomes contained within the epithelial absorptive Cells of the proximal intestine of adult rats. Expand
Ultrastructural localization of nicotinamide adenine dinucleotide phosphatase (NADPase) activity within columnar, goblet, and Paneth cells of rat small intestine.
  • S. Parsons, C. Smith
  • Biology, Medicine
  • The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
  • 1984
NADPase activity within the Golgi saccules could be inhibited completely by incubating intestinal epithelial cells with NADP in the presence of sodium fluoride or L(+)-tartrate. Expand
Clastic cells of Hassall's corpuscles during acute involution of the thymus induced by cyclophosphamide in guinea pigs.
General and histochemical observations of the thymus were carried out in guinea pigs after injection of cyclophosphamide, indicating the dissolution of Hassall's corpuscles by intensive extracellular enzyme release. Expand
Morphologie comparée de la muqueuse intestinale de deux espèces animales aux possibilités d'absorption protéique néonatale différentes
The structure of intestinal absorptive cells of the foetus at term and of the newborn from the first day up to the 20th day of life showed a marked evolution, despite functional differences in neonatal protein absorption. Expand
Histochemistry in psoriasis
  • K. Niizuma
  • Chemistry, Medicine
  • Archives of Dermatological Research
  • 2004
The results suggest that the enzyme which degrades nucleosid-5′-phosphate to nucleoside and inorganic phosphate is not acid phosphatase but 5′-nucleotidase. Expand


Factors governing the validity of staining methods for enzymes, and their bearing upon the Gomori acid phosphatase technique☆
Formol-calcium fixation followed by treatment with hypertonic sucrose containing gum acacia (“gum-sucrose”) gives the best preservation of the acid phosphatase integrity of rat kidney and liver cells. Expand
  • S. J. Holt, R. Hicks
  • Biology, Medicine
  • The Journal of biophysical and biochemical cytology
  • 1961
Analysis of the morphological and biochemical integrity of the fixed tissue suggests that 4 per cent formaldehyde, buffered at pH 7.2 with 0.067 M phosphate, and containing 7.5 per cent sucrose, is the most suitable of the fixatives for combined cytochemical staining and electron microscopical studies. Expand
Relation of Oxygen and Temperature in the Preservation of Tissues by Refrigeration
  • J. H. Hanks, R. Wallace
  • Chemistry, Medicine
  • Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1949
An inquiry has been made into conditions which may influence the viability of 1×1 cm areas of biopsied rabbit skin during refrigeration at 0° and 6-8°C. Since the availability of oxygen, as well asExpand