Demonstration of Usutu virus antibodies in horses, Croatia.

  title={Demonstration of Usutu virus antibodies in horses, Croatia.},
  author={Ljubo Barbi{\'c} and Tatjana Vilibi{\'c}-{\vC}avlek and Eddy Liste{\vs} and Vladimir Stevanovi{\'c} and Ira Gjenero-Margan and Sun{\vc}anica Ljubin-Sternak and Iva Pem-Novosel and Irena Listes and G. Mlinari{\'c}-galinovi{\'c} and Annapia Di Gennaro and Giovanni Savini},
  journal={Vector borne and zoonotic diseases},
  volume={13 10},
We report the first serological evidence of Usutu virus (USUV) infection in horses in Croatia. During 2011, 1380 horse serum samples from healthy animals were collected from six northern Croatian counties. All samples were first screened for West Nile virus (WNV) immunoglobulin G (IgG) antibodies using an enzyme-linked immunosorbent assay (ELISA). Sixty-nine WNV ELISA-reactive samples were further tested for WNV antibodies by a virus neutralization assay (VN assay) and plaque reduction… CONTINUE READING
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