Demonstration of Skin Antibodies in Sera of Pemphigus Vulgaris Patients by Indirect Immunofluorescent Staining.∗

@article{Beutner1964DemonstrationOS,
  title={Demonstration of Skin Antibodies in Sera of Pemphigus Vulgaris Patients by Indirect Immunofluorescent Staining.∗},
  author={Ernst H. Beutner and Robert E. Jordon},
  journal={Proceedings of the Society for Experimental Biology and Medicine},
  year={1964},
  volume={117},
  pages={505 - 510}
}
  • E. Beutner, R. Jordon
  • Published 1 November 1964
  • Biology, Medicine
  • Proceedings of the Society for Experimental Biology and Medicine
Summary 1. Eight of 13 sera from patients with pemphigus vulgaris were found to contain antibodies to a substance at the surface of the cells of stratified squamous epithelium, particularly in the stratum spinosum, as demonstrated by indirect immunofluores-cent (I.I.F.) staining. The reactions of 4 of these 8 sera were deemed to be weakly positive or doubtful while the remaining 4 sera yielded titers of 1:30 to 1:120 by I.I.F. staining. The reactive antigen was found only in stratified squamous… 
AUTOANTIBODIES IN PEMPHIGUS VULGARIS: RESPONSE TO AN INTERCELLULAR SUBSTANCE OF EPIDERMIS.
TLDR
Antibodies to an intercellular substance of stratified squamous epithelium were detected by indirect immunofluorescent (IF) staining in the sera of eight out of 16 patients with pemphigus vulgaris and by direct IF staining of biopsies, γglobulin was demonstrated in the inter cellular areas of the epidermis.
Anti-Intercellular Substance Antibodies: Presence in Serum Samples of 14 Patients Without Pemphigus
TLDR
It seems that anti-ICS antibodies can be produced in several clinical conditions in which there is damage to the epidermis, and may not have any pathogenetic activity in vivo, at least within the time limits defined by the follow-up examinations.
Immunopathologic studies of experimental pemphigus-like autoantibodies and bullous-like lesions.
  • R. Ablin
  • Biology, Medicine
    The Journal of investigative dermatology
  • 1969
TLDR
Heteroimmunization of rabbits with an ethanolic extract of esophageal mucosa incorporating Freund adjuvant has been successful in eliciting pemphigus-like antibodies, which were shown to be autoantibodies by virtue of the demonstration of positive in vitro IF staining reactions with the antibody-producer's own tissues.
AUTOANTIBODIES IN SOME BULLOUS DISEASES
TLDR
'In cases in which, in spite of extensive cutaneous lesions with involvement of the mucous membranes, the antibodies react exclusively with the human or monkey mucosa but not with the epidermis, this appeared to refute their pathogenicity.
Radioimmunoassay to study pemphigus antibodies
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TheRadioimmunoassay demonstrates good clinical correlation and could be effectively used in the clinical management of patients with pemphigus whose sera demonstrate a high binding and Preliminary data indicates that the radioimmunOassay can detect pemPHigus antibodies synthesized in vitro.
Mucosal and mucocutaneous (generalized) pemphigus vulgaris show distinct autoantibody profiles.
TLDR
It is indicated that the autoantibody response in pemphigus vulgaris is heterogeneous, and in one patient the disease evolved from pure mucosal involvement associated with anti-desmoglein 3 antibodies to a disorder involving mucosas and skin.
Epidermal cytoplasmic antibodies
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Antibodies to epidermal cytoplasmic antigens were detected by the indirect immunofluorescence (IF) technique in 36% of 100 adult healthy subjects and in 17.6% of 17 normal newborn infants, postulated that they are the result of an antigenic stimulation by exogenous substances commonly present in the environment.
Detection of class-specific antibodies of pemphigus vulgaris using a solid phase radioimmunoassay.
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A sensitive solid phase radioimmunoassay was developed to detect class-specific pemphigus antibodies and will be useful in studying the cellular mechanisms controlling the synthesis of pemPHigus antibody in vitro.
Molecular mechanisms of pathogenesis.
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Experimental results from several laboratories are described confirming the pathologic events induced in normal human skin by serum or IgG preparations from pemphigus patients and to discuss the molecular events leading to the pathophysiology of pemPHigus.
Direct immunofluorescent studies of pemphigus and bullous pemphigoid.
Direct immunofluorescent (IF) staining was carried out on skin biopsy specimens of patients with skin diseases (including bullous skin diseases), with use of specific antisera to human IgG, IgA, IgM,
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