Demonstration of Functional Coupling between Dopamine Synthesis and Its Packaging into Synaptic Vesicles
@article{Chen2003DemonstrationOF, title={Demonstration of Functional Coupling between Dopamine Synthesis and Its Packaging into Synaptic Vesicles}, author={Rong Chen and Jianning Wei and Stephen C. Fowler and Jang-Yen Wu}, journal={Journal of Biomedical Science}, year={2003}, volume={10}, pages={774 - 781} }
We have previously shown that the membrane-associated form of the GABA-synthesizing enzyme, glutamate decarboxylase 65 (GAD65), is activated by synaptic vesicle proton gradient-mediated protein phosphorylation. We now report that the rate-limiting enzyme in dopamine (DA) biosynthesis, tyrosine hydroxylase (TH), is regulated similarly to GAD65. The membrane-associated form of TH (MTH) was activated by conditions favoring protein phosphorylation (e.g. ATP) and was inhibited by phosphatase (e.g…
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References
SHOWING 1-10 OF 27 REFERENCES
Role of Synaptic Vesicle Proton Gradient and Protein Phosphorylation on ATP-mediated Activation of Membrane-associated Brain Glutamate Decarboxylase*
- Biology, ChemistryThe Journal of Biological Chemistry
- 1999
The membrane-associated form of brain glutamic acid decarboxylase (MGAD) is greatly activated by ATP, whereas adenosine 5′-[β,γ-imido]triphosphate (AMP-PNP), a non-hydrolyzable ATP analog, has no effect on MGAD activity.
Demonstration of functional coupling between γ-aminobutyric acid (GABA) synthesis and vesicular GABA transport into synaptic vesicles
- Biology, ChemistryProceedings of the National Academy of Sciences of the United States of America
- 2003
There is a functional and structural coupling between the synthesis of γ-aminobutyric acid by membrane-associated GAD and its packaging into synaptic vesicles (SVs) by vesicular GABA transporter (VGAT).
Brain L-Glutamate Decarboxylase
- Biology, ChemistryThe Journal of Biological Chemistry
- 1995
Evidence is presented to support the hypothesis that protein kinase A and calcineurin may be involved in regulation of G AD activity through phosphorylation and dephosphorylation of GAD, respectively.
Tyrosine hydroxylase in rat brain dopaminergic nerve terminals. Multiple-site phosphorylation in vivo and in synaptosomes.
- Biology, ChemistryThe Journal of biological chemistry
- 1991
Phosphorylation and Activation of Brain Aromatic l‐Amino Acid Decarboxylase by Cyclic AMP‐Dependent Protein Kinase
- Biology, ChemistryJournal of neurochemistry
- 2000
It is reported that the catalytic subunit of cyclic AMP‐dependent protein kinase (PKA) directly phosphorylated AAAD immunoprecipitated from homogenates prepared from the mouse striatum and midbrain in vitro.
Lack of regulation of aromatic l‐amino acid decarboxylase in intact bovine chromaffin cells
- BiologyJournal of neurochemistry
- 2002
The phosphorylation and activity of AADC was evaluated relative to that of tyrosine hydroxylase (TH) in intact bovine chromaffin cells and showed questionable physiological significance.
Regulatory Properties of Soluble and Particulate Rat Brain Tyrosine Hydroxylase *
- Biology, Chemistry
- 2002
It is suggested that dopamine may be the more significant regulatory metabolite of the striatal biogenic amine biosynthetic pathway, and that tyrosine hydroxylase activity may be regulated by alterations in the physical state of the enzyme.
Dynamic Storage of Dopamine in Rat Brain Synaptic Vesicles In Vitro
- BiologyJournal of neurochemistry
- 1995
It is implied that catecholamines turn over with a half‐time of minutes during active uptake by brain synaptic vesicles in vitro, and the physiological role of uptake‐independent efflux could be to allow the monoamine content of synaptic vESicles to be regulated over a time range of minutes and, thereby, control the amount released by exocytosis.
Identification of protein phosphatase 2A as the major tyrosine hydroxylase phosphatase in adrenal medulla and corpus striatum: evidence from the effects of okadaic acid
- Biology, ChemistryFEBS letters
- 1989
Protein Phosphorylation and Taurine Biosynthesis In Vivo and In Vitro
- Biology, ChemistryThe Journal of Neuroscience
- 1997
It is reported that both in vivo and in vitro the taurine-synthesizing enzyme in the brain, namely cysteine sulfinic acid decarboxylase (CSAD), is activated when phosphorylated and inhibited when dephosphorylated.