Demonstration of Functional Coupling between Dopamine Synthesis and Its Packaging into Synaptic Vesicles

  title={Demonstration of Functional Coupling between Dopamine Synthesis and Its Packaging into Synaptic Vesicles},
  author={Rong Chen and Jianning Wei and Stephen C. Fowler and Jang-Yen Wu},
  journal={Journal of Biomedical Science},
  pages={774 - 781}
We have previously shown that the membrane-associated form of the GABA-synthesizing enzyme, glutamate decarboxylase 65 (GAD65), is activated by synaptic vesicle proton gradient-mediated protein phosphorylation. We now report that the rate-limiting enzyme in dopamine (DA) biosynthesis, tyrosine hydroxylase (TH), is regulated similarly to GAD65. The membrane-associated form of TH (MTH) was activated by conditions favoring protein phosphorylation (e.g. ATP) and was inhibited by phosphatase (e.g… 

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