Demonstration of 1,25(OH)2 vitamin D3 receptors and actions in vascular smooth muscle cellsIn vitro

@article{Merke1987DemonstrationO1,
  title={Demonstration of 1,25(OH)2 vitamin D3 receptors and actions in vascular smooth muscle cellsIn vitro},
  author={Dr. J. Merke and Werner Hofmann and Denise Goldschmidt and Eberhard Ritz},
  journal={Calcified Tissue International},
  year={1987},
  volume={41},
  pages={112-114}
}
Binding of [3H] 1,25 (OH)2D3 and effects of 1,25 (OH)2D3 on cell ultrastructure were evaluated in vascular smooth muscle cells (VSMC) primary cultures (aortic media). Specific reversible binding of [3H] 1,25 (OH)2D3 by a 3.5 S macromolecule with DNA binding, KD 6.2×10−10M and Nmax 16 fmol/mg protein was demonstrated. Incubation of VSMC with 10−8 M 1,25 (OH)2D3, but not 25 (OH)D3, in the presence of 10% FCS for up to three weeks caused rapid reversible appearance in the cytoplasm of membrane… CONTINUE READING

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