Demonstration by a nested PCR for Mycoplasma pneumoniae that M. pneumoniae load in the throat is higher in patients hospitalised for M. pneumoniae infection than in non-hospitalised subjects.

@article{DorigoZetsma1999DemonstrationBA,
  title={Demonstration by a nested PCR for Mycoplasma pneumoniae that M. pneumoniae load in the throat is higher in patients hospitalised for M. pneumoniae infection than in non-hospitalised subjects.},
  author={J. Wendelien Dorigo-Zetsma and Sebastiaan A J Zaat and Aldwin J.M. Vriesema and Jacob B. Dankert},
  journal={Journal of medical microbiology},
  year={1999},
  volume={48 12},
  pages={1115-22}
}
A nested PCR protocol to detect Mycoplasma pneumoniae DNA in throat specimens was developed. An amplification control (AC) template, which is amplified by the same primers as the M. pneumoniae target sequence, was constructed. The assay allowed highly specific and sensitive detection of M. pneumoniae DNA. In all, 305 throat samples, 62 from hospitalised… CONTINUE READING