Demonstration and purification of multiple bovine brain and bovine lung calmodulin-stimulated phosphatase isozymes.

@article{Yokoyama1991DemonstrationAP,
  title={Demonstration and purification of multiple bovine brain and bovine lung calmodulin-stimulated phosphatase isozymes.},
  author={Noriko Yokoyama and Jerry H. C. Wang},
  journal={The Journal of biological chemistry},
  year={1991},
  volume={266 22},
  pages={14822-9}
}
Calmodulin (CaM)-stimulated phosphatase in bovine brain or bovine lung CaM-binding protein fractions were fractionated on a heparin-Sepharose column into three activity peaks, designated in order of column three activity peaks, designated in order of column elution as the brain peak I (BPI), peak II (BPII), and peak III (BPIII) or the lung peak I (LPI), peak II (LPII), and peak III (LPIII) phosphatases, respectively. The pooled individual peak fractions were further purified on a fast protein… CONTINUE READING