Demonstrating plasmid-based horizontal gene transfer in complex environmental matrices: a practical approach for a critical review.

@article{Bellanger2014DemonstratingPH,
  title={Demonstrating plasmid-based horizontal gene transfer in complex environmental matrices: a practical approach for a critical review.},
  author={Xavier Bellanger and H{\'e}l{\`e}ne Guilloteau and S{\'e}bastien Bonot and Christophe Merlin},
  journal={The Science of the total environment},
  year={2014},
  volume={493},
  pages={
          872-82
        }
}
Plasmid-based dissemination of antibiotic resistance genes in environmental microbial communities is a matter of concern for public health, but it remains difficult to study for methodological reasons. In this study, we used the broad host range plasmid pB10 to compare and to point out the main drawbacks of the three different approaches currently used to evaluate plasmid transfer in natural communities. Culture-based selection of transconjugants appeared to be compromised by high prevalence of… CONTINUE READING

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