Demonstrating internal ribosome entry sites in eukaryotic mRNAs using stringent RNA test procedures.

Abstract

The dicistronic assay for internal ribosome entry site (IRES) activity is the most widely used method for testing putative sequences that may drive cap-independent translation initiation. This assay typically involves the transfection of cells with dicistronic DNA test constructs. Many of the reports describing eukaryotic IRES elements have been criticized… (More)

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