Delta-protein kinase C phosphorylation parallels inhibition of nerve growth factor-induced differentiation independent of changes in Trk A and MAP kinase signalling in PC12 cells.

  title={Delta-protein kinase C phosphorylation parallels inhibition of nerve growth factor-induced differentiation independent of changes in Trk A and MAP kinase signalling in PC12 cells.},
  author={Marie W. Wooten and Michael Lamar Seibenhener and Jari E. Heikkila and Harald Mischak},
  journal={Cellular signalling},
  volume={10 4},
We investigated the ability of bryostatin 1 to block nerve growth factor (NGF)-induced differentiation of pheochromocytoma PC12 cells and to effect expression of protein kinase C (PKC) isoforms. Compared with phorbol myristate acetate (PMA), a likewise potent activator of PKC, high doses of bryostatin (> 200 nM) failed to down-regulate delta-PKC, as with zeta-PKC, whereas, alpha-PKC was completely down-regulated. Two forms of delta-PKC were expressed in PC12 cells, a phosphorylated 78.000 M(r… Expand
Selective association of protein kinase C with 14-3-3 zeta in neuronally differentiated PC12 Cells. Stimulatory and inhibitory effect of 14-3-3 zeta in vivo.
The results show that the association with 14-3-3 zeta has distinct effects on classical PKC and non-classical PKC activity, and the immunoprecipitate by anti-FLAG antibody contains constitutive and autonomous Ca(2+)-independent non- classical PKCs activity. Expand
The roles of signaling by the p42/p44 mitogen-activated protein (MAP) kinase pathway; a potential route to radio- and chemo-sensitization of tumor cells resulting in the induction of apoptosis and loss of clonogenicity
During the last 10 years, multiple signal transduction pathways within cells have been discovered. These pathways have been linked to the regulation of many diverse cellular events such asExpand
Neurotrophins Induce Neuregulin Release through Protein Kinase Cδ Activation*
These findings implicate this specific PKC isoform as an important factor for the cleavage and neurotrophin-regulated release of soluble NRG1 forms that have important effects in nervous system development and disease. Expand
Pro‐apoptotic protein kinase Cδ is associated with intranuclear inclusions in a transgenic model of Huntington's disease
It is suggested that the association of PKCδ with intranuclear htt‐aggregates may affect its apoptotic function in a transgenic model of HD and evidence is presented that accumulation of PK Cδ in cell nuclei does not depend on mutant htt nuclear translocation. Expand
Dissecting the Roles of Tyrosines 490 and 785 of TrkA Protein in the Induction of Downstream Protein Phosphorylation Using Chimeric Receptors*
The results from the mutant receptors allowed grouping of these downstream targets by their dependence on the two characterized docking site(s), providing direct evidence that there are other sites on TrkA that are involved in downstream signaling. Expand
Effects of Estrogens and Endocrine-Disrupting Chemicals on Cell Differentiation–Survival–Proliferation in Brain: Contributions of Neuronal Cell Lines
In the present review, some effects of estrogens and EDC are described with focus on ER-mediated effects in neuronal cells, with particular attention to PC12 cells, an interesting model to study the mechanisms underlying ER- mediated differentiating and neuroprotective effects ofEstrogens. Expand
Bryostatin has proved to be a very potent antitumor-promoting agent, and a very promising anticancer drug, and it has provided curative levels of activity against a variety of murine experimental cancer systems. Expand


Inhibition of MAP Kinase Kinase Blocks the Differentiation of PC-12 Cells Induced by Nerve Growth Factor(*)
Data indicate that the MAP kinase pathway is absolutely required for NGF-induced neuronal differentiation in PC-12 cells. Expand
Overexpression of ε-Protein Kinase C Enhances Nerve Growth Factor-induced Phosphorylation of Mitogen-activated Protein Kinases and Neurite Outgrowth (*)
It is demonstrated that ε-PKC promotes NGF-induced neurite outgrowth by enhancing NGF signal transduction, suggesting a role for δ-P KC in neural differentiation and plasticity. Expand
Bryostatin 1 protects protein kinase C-delta from down-regulation in mouse keratinocytes in parallel with its inhibition of phorbol ester-induced differentiation.
It is reported here that in primary mouse keratinocytes PMA caused translocation of PKC-epsilon to the Triton X-100-soluble fraction with an approximately 2-order of magnitude higher potency, compared with translocated ofPKC-alpha and PKD-delta. Expand
A role for zeta protein kinase C in nerve growth factor-induced differentiation of PC12 cells.
Studies were undertaken to compare the signal-induced redistribution of conventional protein kinase C (cPKC) to nonclassical protein kinases C (nP KC) family members in response to phorbol 12-myristate 13-acetate (PMA) or nerve growth factor (NGF) treatment of PC12 cells, and PKC-zeta was found predominantly in the cytoplasm. Expand
The co-mitogenic combination of transforming growth factor beta 1 and bombesin protects protein kinase C-delta from late-phase down-regulation, despite synergy in diacylglycerol accumulation.
It is demonstrated that bombesin plus TGF beta 1 protects PKC-delta from phorbol ester-induced down-regulation, which implies that the down- regulation process itself is regulated. Expand
Tyrosine phosphorylation of protein kinase C-delta in response to its activation.
Tyrosine phosphorylation of purified PKC-delta by src family or receptor tyrosine kinases in vitro enhanced PKC -delta activity, suggesting that tyrosines phosphorylated in vitro may positively affect its function. Expand
A bryostatin-sensitive protein kinase C required for nerve growth factor activity.
The bryostatin-sensitive protein kinase C is necessary, but not sufficient, for neurite outgrowth and acts in the nucleus in a manner independent of c-fos and c-jun transcription. Expand
Nerve growth factor promotes the activation of phosphatidylinositol 3-kinase and its association with the trk tyrosine kinase.
It is demonstrated that NGF activates PtdIns 3-kinase and promotes its association with gp140trk and also show that N GF promotes the tyrosine phosphorylation of the 85-kDa subunit of Ptdins 3-Kinase. Expand
The lack of a role for protein kinase C in neurite extension and in the induction of ornithine decarboxylase by nerve growth factor in PC12 cells.
The data demonstrate that induction of ornithine decarboxylase and formation of neurites in PC12 cells by NGF does not require a protein kinase C-mediated pathway. Expand
Differential regulation of protein kinase C isozymes by bryostatin 1 and phorbol 12-myristate 13-acetate in NIH 3T3 fibroblasts.
It is concluded that bryostatin 1 showed substantially different regulation for PKC alpha, PKC delta, and PKC epsilon, whereas PMA distinguished only weakly between these isozymes. Expand