Delivery of plasmid DNA into intact plant cells by electroporation of plasmolyzed cells

@article{Wu1999DeliveryOP,
  title={Delivery of plasmid DNA into intact plant cells by electroporation of plasmolyzed cells},
  author={F K Wu and T Y Feng},
  journal={Plant Cell Reports},
  year={1999},
  volume={18},
  pages={381-386}
}
This report describes the delivery of plasmid DNA containing either the β-glucuronidase (GUS) or the green fluorescent protein (GFP) reporter gene into intact plant cells of bamboo callus, lilium scales, and Nicotiana benthamiana suspension culture cells. By first plasmolyzing the tissues or cells with 0.4 m sucrose in the presence of plasmid DNA, electroporation effectively delivers plasmid DNA into the intact plant cells. Transient expression of the GUS gene, as revealed by histochemical… CONTINUE READING
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