Delineation of structural domains involved in the subtype specificity of tachykinin receptors through chimeric formation of substance P/substance K receptors.

@article{Yokota1992DelineationOS,
  title={Delineation of structural domains involved in the subtype specificity of tachykinin receptors through chimeric formation of substance P/substance K receptors.},
  author={Yoichi Yokota and Chihiro Akazawa and Hiroaki Ohkubo and Shigetada Nakanishi},
  journal={The EMBO journal},
  year={1992},
  volume={11 10},
  pages={3585-91}
}
The mammalian tachykinin receptors belong to the family of G protein-coupled receptors and consist of the substance P, substance K and neuromedin K receptors (SPR, SKR and NKR). We constructed 14 chimeric receptors in which seven transmembrane segments were sequentially exchanged between the rat SPR and SKR and examined the subtype specificity of the chimeric receptors by radioligand binding and inositol phosphate measurements after transfection into COS cells. All chimeric receptors showed… CONTINUE READING