Delineation of an evolutionary salvage pathway by compensatory mutations of a defective lysozyme.

@article{Jucovi1998DelineationOA,
  title={Delineation of an evolutionary salvage pathway by compensatory mutations of a defective lysozyme.},
  author={M. Jucovi{\vc} and Anthony R. Poteete},
  journal={Protein science : a publication of the Protein Society},
  year={1998},
  volume={7 10},
  pages={2200-9}
}
Model-free approaches (random mutagenesis, DNA shuffling) in combination with more "rational," three-dimensional information-guided randomization have been used for directed evolution of lysozyme activity in a defective T4 lysozyme mutant. A specialized lysozyme cloning vector phage, derived from phage lambda, depends upon T4 lysozyme function for its ability to form plaques. The substitution W138P in T4 lysozyme totally abolishes its plaque-forming ability. Compensating mutations in W138P T4… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.

Similar Papers

Loading similar papers…