• Corpus ID: 42814009

Delineation of IL-5 domains predicted to engage the IL-5 receptor complex.

@article{Dickason1996DelineationOI,
  title={Delineation of IL-5 domains predicted to engage the IL-5 receptor complex.},
  author={R. R. Dickason and Marilyn M. Huston and David P. Huston},
  journal={Journal of immunology},
  year={1996},
  volume={156 3},
  pages={
          1030-7
        }
}
IL-5 is an interdigitating homodimeric glycoprotein and a member of the helical bundle family of cytokines. IL-5 is a potent activator of eosinophils and a specific promoter of their differentiation. This activity has implicated IL-5 in the pathogenesis of asthma and allergic disease. A detailed understanding of IL-5 structure and function is required to develop immunomodulators of IL-5-mediated inflammatory responses. We generated a panel of neutralizing anti-IL-5 mAbs which were used to map… 
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Interleukin-5
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Clinical studies provide insight into the role of IL-5 in health and disease and provide a strong impetus for investigating means of modulating the effects ofIL-5, as well as elucidating the functional structure of Il-5 and its receptor complex, aswell as the mechanisms of IL -5 signal transduction.
Engineering of a functional interleukin-5 monomer: a paradigm for redesigning helical bundle cytokines with therapeutic potential in allergy and asthma
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No differences in signal transduction pathways utilized by mono5 and IL-5 are demonstrated, as determined by western blot analysis of early tyrosine phosphorylation events, Jak2 activation, and mitogen-activated protein kinase activation.
Expression and in vitro properties of guinea pig IL-5: comparison to human and murine orthologs.
TLDR
Differences in affinity for IL-5r orthologs correlated with bioassay results: human TF-1 cells showed roughly comparable proliferative responses to guinea pig, human and murine IL-3 whereas murine B13 cells showed a strong preference for murine over guinea Pig and human IL- 5 (EC50 = 1.9, 2200 and 720 pM, respectively).
Biological and molecular characteristics of interleukin-5 and its receptor.
TLDR
This review covers the major biological, molecular and structural aspects of IL5 research since the identification of this cytokine ten years ago and concludes that IL5 emerges to be an attractive target for the generation of new anti-allergic drugs.
Biology of common β receptor–signaling cytokines: IL-3, IL-5, and GM-CSF
Neutralizing monoclonal antibodies can potentiate IL‐5 signaling
TLDR
It is found that H30 as well as 5A5 can increase proliferation above the level induced by human (h) IL‐5 alone, in a JAK‐2‐dependent manner, and at every sub‐optimal hIL‐5 concentration analyzed.
Mapping and characterization of the epitope(s) of Sch 55700, a humanized mAb, that inhibits human IL-5.
TLDR
It is concluded that Sch 55700 occupies a region, ERRR, on IL-5 that is essential for its interaction with the receptor and thereby blocks IL-4 bioactivity and also proposes a model suggesting that the molecular interactions between theIL-5 and Sch 5 5700 mainly involve several ion pair interactions.
CHAPTER 11 – Interleukin-5
Interleukin-5, a therapeutic target in allergic inflammation.
TLDR
Clinical investigations support a central role for IL-5 in allergic asthma and a role for mast cells in the initial recruitment of eosinophils, and T cell-depletion studies indicate that TH2 cells are the major source ofIL-5.
Th2 cytokines and asthma — The role of interleukin-5 in allergic eosinophilic disease
TLDR
The present review addresses the data that relate interleukin-5 to pulmonary inflammation and function in animal models, and the use of neutralizing anti-interleukIn-5 monoclonal antibodies for the treatment of asthma in humans.
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