Deletion of the omega-loop in the active site of staphylococcal nuclease. 1. Effect on catalysis and stability.

Abstract

The high-resolution X-ray structure of wild-type staphylococcal nuclease (E43 SNase) suggests that Glu 43 acts a general basic catalyst to assist the attack of water on a phosphodiester substrate [Loll, P., & Lattman, E. E. (1989) Proteins: Struct., Funct., Genet. 5, 183]. Glu 43 is located at the base of the solvent-exposed and conformationally mobile… (More)

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Cite this paper

@article{Poole1991DeletionOT, title={Deletion of the omega-loop in the active site of staphylococcal nuclease. 1. Effect on catalysis and stability.}, author={Leslie B. Poole and Deborah A Loveys and Susanne Hale and John A. Gerlt and S M Stanczyk and Philip H. Bolton}, journal={Biochemistry}, year={1991}, volume={30 15}, pages={3621-7} }