Deletion of the gene encoding the reductase component of 3-ketosteroid 9α-hydroxylase in Rhodococcus equi USA-18 disrupts sterol catabolism, leading to the accumulation of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid and 1,4-androstadiene-3,17-dione

Abstract

The gene encoding the putative reductase component (KshB) of 3-ketosteroid 9α-hydroxylase was cloned from Rhodococcus equi USA-18, a cholesterol oxidase-producing strain formerly named Arthrobacter simplex USA-18, by PCR according to consensus amino acid motifs of several bacterial KshB subunits. Deletion of the gene in R. equi USA-18 by a PCR-targeted gene… (More)
DOI: 10.1186/s12934-014-0130-3

Topics

9 Figures and Tables

Cite this paper

@inproceedings{Yeh2014DeletionOT, title={Deletion of the gene encoding the reductase component of 3-ketosteroid 9α-hydroxylase in Rhodococcus equi USA-18 disrupts sterol catabolism, leading to the accumulation of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid and 1,4-androstadiene-3,17-dione}, author={Chin-Hsing Yeh and Yung-Shun Kuo and Che-Ming Chang and Wen-Hsiung Liu and Meei-Ling Sheu and Menghsiao Meng}, booktitle={Microbial cell factories}, year={2014} }