Deletion mutagenesis identifies a haploinsufficient role for γ-zein in opaque2 endosperm modification.

Abstract

Quality Protein Maize (QPM) is a hard kernel variant of the high-lysine mutant opaque2. Using γ-irradiation, we created opaque QPM variants to identify opaque2 modifier genes and to investigate deletion mutagenesis combined with Illumina sequencing as a maize (Zea mays) functional genomics tool. A K0326Y QPM deletion mutant was null for the 27- and 50-kD… (More)
DOI: 10.1104/pp.113.230961

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