Delayed-type hypersensitivity and lymphocyte proliferation in response to Leishmania major infection in a group of children in Jericho.

@article{Alvarado1989DelayedtypeHA,
  title={Delayed-type hypersensitivity and lymphocyte proliferation in response to Leishmania major infection in a group of children in Jericho.},
  author={Ram{\'o}n Alvarado and Claes Enk and Kahena Jaber and Lee Schnur and Shoshana Frankenburg},
  journal={Transactions of the Royal Society of Tropical Medicine and Hygiene},
  year={1989},
  volume={83 2},
  pages={189-92}
}
The cellular response to Leishmania major was evaluated in vitro with a lymphocyte proliferation microtest, performed on 100 microliters of whole blood obtained by finger prick. The maximum time and optimum conditions for storage of fresh blood before testing were determined, and the ability of the assay to evaluate cellular immunity to Leishmania was compared to that of the classical Montenegro skin test. A positive correlation between the diameter of the skin induration and the stimulation… CONTINUE READING

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