Delayed release of inorganic phosphate from elongation factor Tu following GTP hydrolysis on the ribosome.

@article{Kothe2006DelayedRO,
  title={Delayed release of inorganic phosphate from elongation factor Tu following GTP hydrolysis on the ribosome.},
  author={Ute Kothe and Marina V. Rodnina},
  journal={Biochemistry},
  year={2006},
  volume={45 42},
  pages={12767-74}
}
The dissociation of inorganic phosphate (P(i)) following GTP hydrolysis is a key step determining the functional state of many GTPases. Here, the timing of P(i) release from elongation factor Tu (EF-Tu) and its implications for the function of EF-Tu on the ribosome were studied by rapid kinetic techniques. It was found that P(i) release from EF-Tu is >20-fold slower than GTP cleavage and limits the rate of the conformational switch of EF-Tu from the GTP- to the GDP-bound form. The point… CONTINUE READING

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