Delay of migrating leukocytes by the basement membrane deposited by endothelial cells in long-term culture

@inproceedings{Burton2011DelayOM,
  title={Delay of migrating leukocytes by the basement membrane deposited by endothelial cells in long-term culture},
  author={Victoria J. Burton and Lynn M Butler and Helen M McGettrick and Phil C. Stone and Hannah C. Jeffery and Caroline Olivia Susan Savage and G Ed Rainger and Gerard B Nash},
  booktitle={Experimental cell research},
  year={2011}
}
We investigated the migration of human leukocytes through endothelial cells (EC), and particularly their underlying basement membrane (BM). EC were cultured for 20days on 3μm-pore filters or collagen gels to form a distinct BM, and then treated with tumour necrosis factor-α, interleukin-1β or interferon-γ. Neutrophil migration through the cytokine-treated EC and BM was delayed for 20-day compared to 4-day cultures. The BM alone obstructed chemotaxis of neutrophils, and if fresh EC were briefly… CONTINUE READING