Degranulation from human eosinophils stimulated with C3a and C5a.

  title={Degranulation from human eosinophils stimulated with C3a and C5a.},
  author={Shigeru Takafuji and Kenji Tadokoro and Kazuhiro Ito and Clemens Andreas Dahinden},
  journal={International archives of allergy and immunology},
  volume={104 Suppl 1 1},
It is unclear what actually induces eosinophil degranulation in vivo. We examined eosinophil cationic protein (ECP) release from normal human eosinophils (Eos) in response to C3a and C5a. C3a and C5a induced remarkable ECP release when Eos were preincubated with cytochalasin B. ECP release induced by C3a or C5a was greater than that induced by PAF at a concentration of 10(-7) M. The ED50 for ECP release was 3 x 10(-8) M and 3 x 10(-9) M for C3a and C5a, respectively. C3a or C5a elicited a rapid… CONTINUE READING