Degradation of supercoiled plasmid DNA within a capillary device.

  title={Degradation of supercoiled plasmid DNA within a capillary device.},
  author={Francis J Meacle and Hu Zhang and Ioannis Papantoniou and John M Ward and Nigel John Titchener-Hooker and M. J. Hoare},
  journal={Biotechnology and bioengineering},
  volume={97 5},
Supercoiled plasmid DNA is susceptible to fluid stress in large-scale manufacturing processes. A capillary device was used to generate controlled shear conditions and the effects of different stresses on plasmid DNA structure were investigated. Computational fluid dynamics (CFD) analysis was employed to characterize the flow environment in the capillary device and different analytical techniques were used to quantify the DNA breakage. It was found that the degradation of plasmid DNA occurred at… CONTINUE READING

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