Degradation of neurofilament proteins by purified human brain cathepsin D.

@article{Nixon1984DegradationON,
  title={Degradation of neurofilament proteins by purified human brain cathepsin D.},
  author={Ralph A. Nixon and Charles A. Marotta},
  journal={Journal of neurochemistry},
  year={1984},
  volume={43 2},
  pages={507-16}
}
Cathepsin D (CD) was purified to homogeneity from postmortem human cerebral cortex. Incubation of CD with human neurofilament proteins (NFPs) prepared by axonal flotation led to the rapid degradation of the 200,000, 160,000, and 70,000 NFP subunits (200K, 160K, and 70K) which had been separated by one- or two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Degradation was appreciable at enzyme activity-to-substrate protein ratios that were two- to threefold… CONTINUE READING