Degradation of melphalan in vitro: rationale for the use of continuous exposure in chemosensitivity assays

@article{Bosanquet1988DegradationOM,
  title={Degradation of melphalan in vitro: rationale for the use of continuous exposure in chemosensitivity assays},
  author={Andrew G. Bosanquet and Martin C. Bird},
  journal={Cancer Chemotherapy and Pharmacology},
  year={1988},
  volume={21},
  pages={211-215}
}
The hydrolysis of melphalan in cell culture medium at 37° C has been studied. Degradation of melphalan proceeded via monohydroxy-melphalan (MOH) to dihydroxymelphalan [M(OH)2] with a half-life of 66 min for melphalan and 58 min for MOH. The half-life for melphalan was similar to the terminal half-life of the drug in vivo. The effect of the two metabolites, MOH and M(OH)2, on the chemosensitivity of K562 leukaemia cells during continuous exposure to melphalan was also examined. M(OH)2 had no… CONTINUE READING